Method development and validation for acrylamide in potato cutlet by UHPLC-MS/MS

Abstract

Surge in consumption of healthy and safe foods has challenged researcher to develop sensitive, precise, robust detection and quantification of food contaminants like acrylamide even if they are present in trace. Keeping this in view, a robust and sensitive analytical method was developed and validated for acrylamide quantification in a potato-based food product (potato cutlet), using LC-MSMS with positive electrospray ionization (+ESI). The method consists of adding acrylamide-d3 (deuterium labelled acrylamide) as an internal standard, extraction by modified QuEChERS method with d-SPE clean-up. Extracts were run on a reverse phase C-18 column for analysis by liquid chromatography. The limit of detection (LOD) and limit of quantification (LOQ) were determined as 0.7 and 2.0 ?g kg?1, respectively, demonstrating the sensitivity of the method for trace detection. The developed method showed excellent results in terms of recoveries (91.0-109.16%), repeatability (RSD 1.8-10.60%), reproducibility (RSD 2.3-11.24%) and robustness (RSD 1.74-4.54%). The repeatability, reproducibility and robustness respectively, show the consistency, accuracy and stability of the developed method. The deviations in ion ratio and retention time were 17.25% and ?0.01 min, respectively. Compared to previous findings, the current study has achieved lower LOD and LOQ levels that reveal the higher sensitivity of this method for acrylamide quantification in potato cutlet.