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Exploring CD26-/lo subpopulations of lymphocytes in asthma phenotype and severity: A novel CD4+ T cell subset expressing archetypical granulocyte proteins.

dc.contributor.authorVázquez-Mera, Sara
dc.contributor.authorMartelo Vidal, Laura
dc.contributor.authorMiguéns Suárez, Pablo
dc.contributor.authorBravo López, Susana Belén
dc.contributor.authorSaavedra Nieves, Paula
dc.contributor.authorArias Crespo, Pilar
dc.contributor.authorFerreiro Posse, Antía
dc.contributor.authorVázquez Lago, Juan Manuel 
dc.contributor.authorSalgado Castro, Francisco Javier
dc.contributor.authorGonzález Barcala, Francisco Javier 
dc.contributor.authorNieto Fontarigo, Juan José
dc.date.accessioned2025-10-20T09:59:12Z
dc.date.available2025-10-20T09:59:12Z
dc.date.issued2024-11
dc.identifier.otherhttps://pubmed.ncbi.nlm.nih.gov/39319599/es
dc.identifier.urihttp://hdl.handle.net/20.500.11940/22042
dc.description.abstract[EN] Asthma pathology may induce changes in naïve/memory lymphocyte proportions assessable through the evaluation of surface CD26 (dipeptidyl peptidase 4/DPP4) levels. Our aim was to investigate the association of asthma phenotype/severity with the relative frequency of CD26-/lo, CD26int and CD26hi subsets within different lymphocyte populations. The proportion of CD26-/lo, CD26int and CD26hi subsets within CD4+ effector T cells (Teff), total CD4- lymphocytes, γδ-T cells, NK cells and NKT cells was measured in peripheral blood samples from healthy (N = 30) and asthma (N = 119) donors with different phenotypes/severities by flow cytometry. We performed K-means clustering analysis and further characterised the CD4+CD26-/lo Teff cell subset by LC-MS/MS and immunofluorescence. Cluster analysis including clinical and flow cytometry data resulted in four groups, two of them with opposite inflammatory profiles (neutrophilic vs. eosinophilic). Neutrophilic asthma presented reduced CD4-CD26hi cells, which negatively correlated with systemic inflammation. Eosinophilic asthma displayed a general expansion of CD26-/lo subsets. Specifically, CD4+CD26-/lo Teff expansion was confirmed in asthma, especially in atopic patients. Proteomic characterisation of this subset with a TEM/TEMRA phenotype revealed upregulated levels of innate (e.g. MPO and RNASE2) and cytoskeleton/extracellular matrix (e.g. MMP9 and ACTN1) proteins. Immunofluorescence assays confirmed the presence of atypical proteins for CD4+ T cells, and an enrichment in 'flower-like' nuclei and MMP9/RNASE2 levels in CD4+CD26-/lo Teff compared to CD4+ T lymphocytes. There is an association between CD26 levels in different lymphocyte subsets and asthma phenotype/severity. CD4+CD26-/loTEMRA cells expressing innate proteins specific to eosinophils/neutrophils could be determinant in sustaining long-term inflammation in adult allergic asthma.es
dc.language.isoenges
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.meshAsthma *
dc.subject.meshImmunophenotyping *
dc.subject.meshEosinophils *
dc.subject.meshT-Lymphocyte Subsets *
dc.subject.meshAdult *
dc.subject.meshFlow Cytometry *
dc.subject.meshMemory *
dc.subject.meshForced Expiratory Volume *
dc.subject.meshMiddle Aged *
dc.subject.meshDipeptidyl Peptidase 4 *
dc.subject.meshPhenotype *
dc.subject.meshSeverity of Illness Index *
dc.subject.meshGranulocytes *
dc.subject.meshCD4-Positive T-Lymphocytes *
dc.titleExploring CD26-/lo subpopulations of lymphocytes in asthma phenotype and severity: A novel CD4+ T cell subset expressing archetypical granulocyte proteins.es
dc.typeArtigoes
dc.identifier.doi10.1111/all.16327
dc.identifier.essn1398-9995
dc.identifier.pmid39319599
dc.issue.number11es
dc.journal.titleAllergyes
dc.organizationServizo Galego de Saúde::Áreas Sanitarias (A.S.)::Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS)es
dc.organizationServizo Galego de Saúde::Áreas Sanitarias (A.S.)::Área Sanitaria de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Medicina Preventivaes
dc.organizationServizo Galego de Saúde::Áreas Sanitarias (A.S.)::Área Sanitaria de Santiago de Compostela - Complexo Hospitalario Universitario de Santiago de Compostela::Neumoloxíaes
dc.page.initial3005es
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/epdf/10.1111/all.16327es
dc.rights.accessRightsopenAccesses
dc.subject.decslinfocitos T CD4-positivos *
dc.subject.decsíndice de gravedad de la enfermedad *
dc.subject.decsmediana edad *
dc.subject.decsfenotipo *
dc.subject.decsinmunofenotipificación *
dc.subject.decsdipeptidil peptidasa-4 *
dc.subject.decssubgrupos de linfocitos T *
dc.subject.decsadulto *
dc.subject.decsasma *
dc.subject.decsgranulocitos *
dc.subject.decseosinófilos *
dc.subject.decscitometría de flujo *
dc.subject.decsvolumen espiratorio forzado *
dc.subject.decsmemoria *
dc.subject.keywordCHUSes
dc.subject.keywordIDISes
dc.typefidesArtigo Científico (inclue Orixinal, Orixinal breve, Revisión Sistemática e Meta-análisis)es
dc.typesophosArtículo Originales
dc.volume.number79es


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Allergy. 2024 Nov;79(11):3005-3021

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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